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antibody goat polyclonal anti ephb2  (R&D Systems)


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    Structured Review

    R&D Systems antibody goat polyclonal anti ephb2
    Antibody Goat Polyclonal Anti Ephb2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+ephb2/Human%2FMouse+EphB2+Antibody/bio_rxiv__64898__2026__04__20__719620-201-5-11
    Average 93 stars, based on 104 article reviews
    antibody goat polyclonal anti ephb2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Role of EphB2/ephrin-B1 signalling in the development and progression of obesity-associated colorectal cancer
    Article Snippet: The following antibodies were used: goat anti-EphB2 (1:200; R&D systems, Minneapolis, MN, USA), goat anti-ephrinB1 (1:400; R&D systems), rat anti-Macrophage (1:30; BMA Biomedicals, Rheinstrasse, Switzerland), goat anti-monocyte chemoattractant protein-1 (MCP-1) (1:100; R&D Systems), and rat anti-diphosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2) (1:100; Sigma-Aldrich, St. Louis, MO, USA).

    Article Title: EPH Receptor Tyrosine Kinases Regulate Epithelial Morphogenesis and Phosphorylate the PAR-3 Scaffold Protein to Modulate Downstream Signaling Networks
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Millipore, #05-321), mouse anti-GFP (Abcam, #290), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), goat anti-EPHA1 (R&D Systems, #AF638), mouse anti-EPHA2 (Millipore, #05-480), goat anti-EPHB4 (R&D Systems, #AF3038), mouse anti-PKCι (BD, #610175), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07-330), mouse anti-actin (Cell Signaling Technology, #3700).

    Article Title: EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.
    Article Snippet: Primary antibodies used were: rabbit anti-FLAG (Sigma-Aldrich, #F7425), mouse anti-pan phosphotyrosine 4G10 (Sigma-Aldrich, #05–321), mouse anti-GFP (Thermo Fisher Scientific, #A-11122, Roche, #1181446001), mouse anti-EPHA4 (BD Biosciences, #610471), goat anti-EPHB2 (R&D Systems, # AF467), rabbit anti-CSK (Cell Signaling Technology, #4980), rabbit anti-PAR-3 (Millipore, #07–330), rabbit anti-LIMK2 (Cell Signaling Technology, #3845), rabbit anti-phospho-SRC (Tyr527) (Cell Signaling Technology, #2105), mouse anti-SRC (Cell Signaling Technology, #2110) mouse anti-Actin (Cell Signaling Technology, #3700), mouse anti-Tubulin (Cell Signaling Technology, #3873).

    Incubation:

    Article Title: EphB2 receptor promotes dermal fibrosis in systemic sclerosis.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 5% donkey serum in 1x Tris-buffered-saline containing 0.1% Triton-X-100 (TBS-T) for 1h at room temperature and incubated overnight at 4 °C with goat anti-EphB2 (R&D System), rabbit anti-phosphoEphB1/B2Y594 + Y596 (Abcam), and mouse anti-αSMA (1:200 dilution in 1% BSA TBS-T) antibodies. ..

    Article Title: EPHB2 promotes diet-induced MASH liver fibrosis
    Article Snippet: Paraffin-embedded sections of human liver were deparaffinized, rehydrated, and treated for 20 min with boiling citrate antigen retrieval buffer (Sigma-Aldrich). .. Slides were blocked with 5% donkey serum in 1x Tris-buffered-saline containing 0.1% Triton-X-100 (TBS-T) for 1h at room temperature and incubated overnight at 4 °C with goat anti-EphB2 (AF467, R&D System), rabbit anti-phosphoEphB1/B2 Y594 + Y596 (Abcam), and mouse anti-αSMA (1:200 dilution in 1% BSA TBS-T) antibodies. ..

    Article Title: Muscarinic receptor M3 contributes to intestinal stem cell maintenance via EphB/ephrin-B signaling
    Article Snippet: .. Sections were then incubated with goat anti-EphB2 (1:50; R&D Systems), goat anti-ephrin-B1 (1:50; R&D Systems), and rabbit anti-ephrin-B2 (1:50; R&D Systems) overnight at 4°C. ..

    SDS Page:

    Article Title: EphB2 mediates social isolation-induced memory forgetting
    Article Snippet: .. Bound proteins were separated by SDS PAGE, transferred to nitrocellulose membranes, and immunoblotted with the indicated antibodies: goat anti-EphB1 (1:500, Santa Cruz Biotechnology, sc-68317), goat anti-EphB2 (1:1000, R&D, P54763), mouse anti-synaptophysin (1:1000, Abcam, ab8049), rabbit anti-PSD95 (1:1000; Cell Signaling Technology, 3450), mouse anti-β-actin (1:3000; Thermo Fisher Scientific, MA5-15739), rabbit anti-GluN1 (1:1000, BD, 556308), mouse anti-GluA1 (1:1000, Santa Cruz Biotechnology, sc-13152), GluA2 (1:1000, Santa Cruz Biotechnology, sc-7611), GluA6 (1:1000, Santa Cruz Biotechnology, sc-7618), rabbit anti-GluN2A (1:1000, Millipore, ab1555P), and mouse anti-GluN2B (1:1000, BD, 610417). ..

    Sample Prep:

    Article Title: Hepatocytes differentiate into intestinal epithelial cells through a hybrid epithelial/mesenchymal cell state in culture
    Article Snippet: .. Methodology Sample preparation Technology, 3195S, 1:500), Mouse anti-E-cad (BD Biosciences, 610182, 1:1000), Mouse anti-Vim (Sigma-Aldrich, V5255, 1:1000), Rabbit anti-Fah (Abcam, ab151998, 1:2000), Mouse anti-Cdx2 (MBL, MU392AUC, 1:100), Rabbit anti-Sox9 (Millipore, AB5535, 1:1000), Goat anti-Villin (Santa Cruz Biotechnology, SC-7672, 1:1000), Rabbit anti-Ki67 (Abcam, ab15580, 1:500), Rabbit anti-Muc2 (Santa Cruz Biotechnology, SC-15334, 1:1000), Rabbit anti-Klf5 (GeneTex, GTX103289, 1:100), Goat anti-EphB2 (R&D Systems, AF467, 1:500), Rabbit anti-Lyz (Dako, A0099, 1:1000), Goat anti-ChgA (Santa Cruz Biotechnology, SC-1488, 1:500), Rabbit anti-CC3 (Cell Signaling Technology, 9661, 1:500), Rabbit anti-YAP (Cell Signaling Technology, 14074, 1:500), Rabbit anti-pHH3 (Millipore, 06-570, 1:500), Rabbit anti-GFP (MBL, 598, 1:500), Normal mouse IgG (Santa Cruz Biotechnology, sc-2025, 1:100 or 1:1000), Normal rabbit IgG (Santa Cruz Biotechnology, sc-2027, 1:500 or 1:1000), Normal goat IgG (Santa Cruz Biotechnology, sc-2028, 1:1000), Guinea pig anti-CK8/18 (Progen, GP11, 1:500), and Mouse anti-!SMA (Sigma-Aldrich, A2547, 1:2000). .. Secondary antibodies: Alexa 488- conjugated donkey anti-rabbit IgG (Invitrogen, A21206, 1:2000), Alexa 488-conjugated donkey anti-goat IgG (Invitrogen, A11055, 1:2000), Alexa 488-conjugated donkey anti-mouse IgG (Invitrogen, A21202, 1:2000), Alexa 488-conjugated goat anti-mouse IgM (Invitrogen, A21042, 1:2000), Alexa 555-conjugated donkey anti-rabbit IgG (Invitrogen, A31572, 1:2000), Alexa 555-conjugated donkey anti-goat IgG (Invitrogen, A21432, 1:2000), Alexa 555-conjugated donkey anti-mouse IgG (Invitrogen, A31570, 1:2000), Alexa 555-conjugated goat anti-mouse IgM (Invitrogen, A21426, 1:2000), Alexa 555-conjugated goat anti-Guinea Pig IgG (Invitrogen, A21435, 1:2000), and HRP-conjugated goat anti-rabbit IgG (Dako, P0448, 1:2000).



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    Fig. 5. LGR5 and <t>EphB2</t> protein expression in differentiated colonoids in response to tofacitinib or budesonide treatment. (a) Immunoblots showing LGR5 and EphB2 protein expression in colonoids from N = 6 donors (4HC and 2UC) cultured at 2% oxygen. Original full blot images are presented in the Supplementary Figure S1. (b,c) Quantification of LGR5 and EphB2 at group level in response to different treatments (1–6) (d,e) Paired quantification of LGR5 and EphB2 protein expression between selected treatments. The colonoids were pre-treated with tofacitinib or budesonide for 3 days alone or before TNF + Poly(I:C) (see Fig. 1). The x-axis shows the treatment, and the y-axis shows fold induction. Fold expression is generated by normalizing to vehicle control (DMSO 0.033%) for each donor and further normalized to GAPDH expression. Differences were evaluated using paired t-test. *P < 0.05.
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    Fig. 5. LGR5 and <t>EphB2</t> protein expression in differentiated colonoids in response to tofacitinib or budesonide treatment. (a) Immunoblots showing LGR5 and EphB2 protein expression in colonoids from N = 6 donors (4HC and 2UC) cultured at 2% oxygen. Original full blot images are presented in the Supplementary Figure S1. (b,c) Quantification of LGR5 and EphB2 at group level in response to different treatments (1–6) (d,e) Paired quantification of LGR5 and EphB2 protein expression between selected treatments. The colonoids were pre-treated with tofacitinib or budesonide for 3 days alone or before TNF + Poly(I:C) (see Fig. 1). The x-axis shows the treatment, and the y-axis shows fold induction. Fold expression is generated by normalizing to vehicle control (DMSO 0.033%) for each donor and further normalized to GAPDH expression. Differences were evaluated using paired t-test. *P < 0.05.
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    Fig. 5. LGR5 and <t>EphB2</t> protein expression in differentiated colonoids in response to tofacitinib or budesonide treatment. (a) Immunoblots showing LGR5 and EphB2 protein expression in colonoids from N = 6 donors (4HC and 2UC) cultured at 2% oxygen. Original full blot images are presented in the Supplementary Figure S1. (b,c) Quantification of LGR5 and EphB2 at group level in response to different treatments (1–6) (d,e) Paired quantification of LGR5 and EphB2 protein expression between selected treatments. The colonoids were pre-treated with tofacitinib or budesonide for 3 days alone or before TNF + Poly(I:C) (see Fig. 1). The x-axis shows the treatment, and the y-axis shows fold induction. Fold expression is generated by normalizing to vehicle control (DMSO 0.033%) for each donor and further normalized to GAPDH expression. Differences were evaluated using paired t-test. *P < 0.05.
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    Fig. 5. LGR5 and <t>EphB2</t> protein expression in differentiated colonoids in response to tofacitinib or budesonide treatment. (a) Immunoblots showing LGR5 and EphB2 protein expression in colonoids from N = 6 donors (4HC and 2UC) cultured at 2% oxygen. Original full blot images are presented in the Supplementary Figure S1. (b,c) Quantification of LGR5 and EphB2 at group level in response to different treatments (1–6) (d,e) Paired quantification of LGR5 and EphB2 protein expression between selected treatments. The colonoids were pre-treated with tofacitinib or budesonide for 3 days alone or before TNF + Poly(I:C) (see Fig. 1). The x-axis shows the treatment, and the y-axis shows fold induction. Fold expression is generated by normalizing to vehicle control (DMSO 0.033%) for each donor and further normalized to GAPDH expression. Differences were evaluated using paired t-test. *P < 0.05.
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    Image Search Results


    Fig. 5. LGR5 and EphB2 protein expression in differentiated colonoids in response to tofacitinib or budesonide treatment. (a) Immunoblots showing LGR5 and EphB2 protein expression in colonoids from N = 6 donors (4HC and 2UC) cultured at 2% oxygen. Original full blot images are presented in the Supplementary Figure S1. (b,c) Quantification of LGR5 and EphB2 at group level in response to different treatments (1–6) (d,e) Paired quantification of LGR5 and EphB2 protein expression between selected treatments. The colonoids were pre-treated with tofacitinib or budesonide for 3 days alone or before TNF + Poly(I:C) (see Fig. 1). The x-axis shows the treatment, and the y-axis shows fold induction. Fold expression is generated by normalizing to vehicle control (DMSO 0.033%) for each donor and further normalized to GAPDH expression. Differences were evaluated using paired t-test. *P < 0.05.

    Journal: Scientific reports

    Article Title: Tofacitinib and budesonide treatment affect stemness and chemokine release in IBD patient-derived colonoids.

    doi: 10.1038/s41598-025-86314-2

    Figure Lengend Snippet: Fig. 5. LGR5 and EphB2 protein expression in differentiated colonoids in response to tofacitinib or budesonide treatment. (a) Immunoblots showing LGR5 and EphB2 protein expression in colonoids from N = 6 donors (4HC and 2UC) cultured at 2% oxygen. Original full blot images are presented in the Supplementary Figure S1. (b,c) Quantification of LGR5 and EphB2 at group level in response to different treatments (1–6) (d,e) Paired quantification of LGR5 and EphB2 protein expression between selected treatments. The colonoids were pre-treated with tofacitinib or budesonide for 3 days alone or before TNF + Poly(I:C) (see Fig. 1). The x-axis shows the treatment, and the y-axis shows fold induction. Fold expression is generated by normalizing to vehicle control (DMSO 0.033%) for each donor and further normalized to GAPDH expression. Differences were evaluated using paired t-test. *P < 0.05.

    Article Snippet: The following antibodies were used: LGR5 Mouse mAb (1:1000, #MA5-25644, Invitrogen), EPH Receptor B2 (EphB2) Goat mAb (1:1000 dilution, #AF467, R&D Systems), Phospho-MLKL (Ser358) (E7G7P) Rabbit mAb (1:1000 dilution, #18640, Cell Signaling Technology), MLKL (E7V4W) Mouse mAb (1:1000 dilution, #26539, Cell Signaling Technology), Caspase-3/cleaved caspase-3 (D3R6Y) Rabbit mAb (1:1000 dilution, #14220, Cell Signaling Technology), and GAPDH (D16H11) XP rabbit monoclonal antibody (1:5000 dilution, #5174, Cell Signaling Technology).

    Techniques: Expressing, Western Blot, Cell Culture, Generated, Control